Individual silver grains in the film are visualized with dark field microscopy.
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The dark field microscopy is able to show them, says Enderlein.
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Dark field microscopy is a technique for improving the contrast of unstained, transparent specimens.
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One of the most common ways include visualizing the organisms by immunofluorescence and dark field microscopy.
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Diagnosis is often by blood tests; however, the bacteria can also be seen using dark field microscopy.
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This introduced an illumination scheme into fluorescence microscopy, which has already been used successfully for dark field microscopy under the name ultramicroscopy.
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Dark field microscopy initially had the finding that the spirochete has a corkscrew appearance and that it is spirillar and gram (-) bacteria.
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Dark field microscopy is useful to enhance contrast in unstained samples, but live blood analysis is not proven to be useful for any of its claimed indications.